Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.

نویسندگان

  • F Kirpekar
  • E Nordhoff
  • K Kristiansen
  • P Roepstorff
  • A Lezius
  • S Hahner
  • M Karas
  • F Hillenkamp
چکیده

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

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عنوان ژورنال:
  • Nucleic acids research

دوره 22 19  شماره 

صفحات  -

تاریخ انتشار 1994